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this is an instructional video for creating a dried blood spot card all I hope you will find this video helpful you should always follow your institutions policies and procedures for handling biological specimens before spotting the card provides a patients name or subject ID as appropriate along with the date of birth and collection date on the card fold the cover away from the filter paper and be careful not to touch the specimen collection area you should always wear powder free disposable gloves when handling the card and specimens set a handheld pipette to 75 microliters the tube applied can be kept at room temperature while you prepare the card and set the pipette volume gently invert the collection to five to seven times to ensure the additive is mixed with the patients blood carefully remove the stopper from the blood tube to avoid spilling using the handheld pipette slowly draw up 75 microliters of blood hold the pipette tips steadily a few millimeters above the center of a