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[Music] thank you [Music] the overall goal of this procedure is to provide a standardized protocol for preparing and processing dried blood spots to be subsequently used in immunoassays and in molecular techniques this is accomplished by first taking capillary blood and transferring it to a filter card the second step is to dry blood spots overnight and to obtain six millimeter punches out of the circles of the filter card next the illusion of dried blood from the punches is achieved by immersion in buffer on a Shaker for a minimum of four hours the hemolytic eluates are then freed from debris by centrifugation the final step is to analyze the cleared eluates for serological and molecular markers of infections with the Hepatitis B hepatitis C and human immunodeficiency viruses by commercially available immunoassays and molecular techniques the idea of using plot collected on a filter card made of cellulose originated a century ago nevertheless people using this method for the first ti
