Blot space in ASC

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Upload your ASC file into your DocHub account.
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Once finished, click Download/Export and save your ASC to your device or cloud storage.
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How to blot space in ASC

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What is the mechanism of action of NLRP3 inhibitor?

It also recruits ASC and caspase-1 to form the NLRP3 inflammasome, which mediates GSDMD to cause cell membrane rupture and release IL-1 and IL-18, causing inflammation and pyroptosis. NLRP3 Inflammasome inhibitors target the priming and activation step in the NLRP3 inflammasome signaling pathway.

What has been proposed that activation of the inflammasome is triggered by?

It is mostly triggered by ATP, pore-forming toxins, viral RNA and particulate matter [6,12,13]. Additionally, several molecular and cellular events have been proposed to describe the second step of inflammasome activation.

What are ASC specks?

ASC specks are micrometer-sized structures formed by the inflammasome adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD). ASC specks are characteristic of inflammasome activation. The inflammasome adapter ASC is localized mainly in the nucleus of macrophages and monocytes.

What activates the NLRP3 inflammasome?

K+ efflux. Early studies have shown that K+ efflux is a common phenomenon that activates the NLRP3 inflammasome. NLRP3 agonists first trigger cell membrane permeation of K+ and Na+, which in turn activates the NLRP3 inflammasome.

What is an ASC inflammasome?

Apoptosis-associated speck-like protein containing a CARD (ASC) has a pivotal role in inflammasome assembly and activation. While ASC function has been primarily implicated in innate immune cells, its contribution to lymphocyte biology is unclear.

What receptors activate the inflammasomes?

The inflammasome sensors are categorized based on their structural characteristics into NLRs, ALRs, and pyrin. These receptors possess the ability to form inflammasomes and trigger caspase-1 activation.

What is apoptosis associated speck-like protein ASC?

PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. It is localized mainly in the nucleus of monocytes and macrophages.

How is the NLRP3 inflammasome activated?

The NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome is a cytoplasmic supramolecular complex that is activated in response to cellular perturbations triggered by infection and sterile injury.

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