Blot sigil in ASC

The best way to blot sigil in ASC

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How to blot sigil in ASC

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What activates the NLRP3 inflammasome?

K+ efflux. Early studies have shown that K+ efflux is a common phenomenon that activates the NLRP3 inflammasome. NLRP3 agonists first trigger cell membrane permeation of K+ and Na+, which in turn activates the NLRP3 inflammasome.

What is ASC in inflammation?

Apoptosis-associated speck-like protein containing a CARD (ASC) has a pivotal role in inflammasome assembly and activation. While ASC function has been primarily implicated in innate immune cells, its contribution to lymphocyte biology is unclear.

What is the size of ASC specks?

ASC specks can be observed as they docHub a size of around 1 m and in most cells only one speck forms upon inflammasome activation. Hence, ASC speck formation can be used as a simple upstream readout for inflammasome activation.

What are ASC specks?

ASC specks are micrometer-sized structures formed by the inflammasome adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD). ASC specks are characteristic of inflammasome activation. The inflammasome adapter ASC is localized mainly in the nucleus of macrophages and monocytes.

What are NLRP3 ASC specks an experimental progress of 22 years of inflammasome research?

Speck assembly is the hallmark of NLRP3 inflammasome activation. The 1m structure comprising of NLRP3 and ASC is the first observable phenotype of NLRP3 activation. While the common consensus is that the specks are the site of inflammasome activity, no direct experimental evidence exists to support this notion.

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