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foreign this method can help answer key questions in the neuropharmological field the main advantage of this technique is that itamp;#39;s simple reliable and reproducible to form with amyloid oligomer visual demonstration of this method is critical as the steps of preparing a beta oligma are difficult to learn first adjust the concentration of a beta monomer solution to 0.25 milligrams per milliliter by adding 900 microliters of double distilled water to the tube then continue evaporating the monomer solution with high Purity nitrogen gas until the volume docHubes about 850 microliters with a concentration of about 0.29 milligrams per milliliter so presence of hfip affects the formation of a beta oligma therefore evaporating as much as possible is the critical step of this procedure next use double distilled water to dilute the curcumin stock solution to prepare a working solution of 0.2 and 2 micromolar maintain a ratio of 1 to 1 and mix the a beta solution with curcumin to a