Many people find the process to blot side in xht quite challenging, particularly if they don't often work with paperwork. However, these days, you no longer have to suffer through long guides or spend hours waiting for the editing software to install. DocHub allows you to edit forms on their web browser without setting up new programs. What's more, our feature-rich service provides a complete set of tools for professional document management, unlike so many other online tools. That’s right. You no longer have to donwload and re-upload your templates so frequently - you can do it all in one go!
No matter what type of document you need to update, the process is straightforward. Take advantage of our professional online service with DocHub!
in this video we will show you how to set up a polyacrylamide gel for wet tank transfer in a mini transplot cell after you have removed your gel from the gel cassette equilibrate the gel in a tray with transfer buffer for 15 minutes on a rocking platform pre-soak blotting paper and fiber pads in the transfer buffer and ensure complete coverage if using a nitrocellulose membrane pre-wet the membrane and transfer buffer if using a pbdf membrane pre-soak in 100 methanol to make the blotting sandwich place the gel holder cassette with the black side down and a tray with sufficient transfer buffer place one pre-wet fiber pad onto the black plastic followed by a pre-wet sheet of blotting paper take the gel and carefully place it onto the blotting paper being careful to avoid trapping excessive air bubbles now place your pre-soaked membrane onto the gel using a roller remove any air bubbles between the gel and the membrane to ensure maximum and even protein transfer place a second she
