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[Music] in this video we will show you how to assemble a blotting sandwich for the transplot sd semi-dry transfer cell after you have removed your gel from the cassette equilibrate the gel in a tray with transfer buffer for 15 minutes on a rocking platform this facilitates the removal of salts and detergents if not removed the excess salts will cause heat buildup during transfer next wet the membrane in tbst if using nitrocellulose or methanol if using pvdf by slowly sliding it in at a 45 degree angle allow it to soak for 15 to 20 minutes abrupt wetting can lead to entrapment of air bubbles in the matrix affecting transfer quality completely saturate two pieces of extra thick filter paper and transfer buffer remove the safety cover and cathode assembly from the instrument place a pre-soaked piece of filter paper onto the platinum anode next place the pre-wet membrane on top of the filter paper roll out any air bubbles carefully place the equilibrated gel on top make sure to minimize m
