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hi my name is David Borden Im an RD staff scientist in protein analysis and were going to go through the steps today of the traditional Western blot transfer okay so to get started lets talk about the things we need here first of all so weve got our deionized water weve got our transfer buffer thats already made up into the appropriate formulation weve got our new page gel weve already run our protein samples on weve got our pee PDF filter sandwich kit weve got our sponges weve got our blot module our power supply and a timer okay so lets get started so first of all we need to go ahead and start with our stack assembly so we take two of our sponges and dip them into some of the transfer buffer that Ive already poured into this plastic container next we take what are our filter paper and submerge them both of them in the transfer buffer as well now what Im going to do here is Im going to go ahead and take one of the pre wetted transfer or the one thats because we wanted