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hello everyone and welcome to todays webinar detecting low abundance proteins in a Western blot overcoming challenges for improved signal-to-noise of low expressing and precious sample targets Im Suzy Valdez of lab routes and Ill be your moderator for todays event todays educational web seminar is presented by lab roots and brought to you by thermo Fisher Scientific to learn more about our sponsor please visit them at WWF Fisher comm now lets get started before we begin Id like to remind everyone that this event is interactive we encourage you to participate by submitting as many questions as you want at any time you want during the presentation to do so simply type them into the ask a question box and click send well answer as many questions as we have time for at the end of the presentation if you have any trouble seeing or hearing the presentation click on the support tab found at the top right of the presentation window or report your problems by clicking on the ask a ques