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so in this quick example question were wanting to look at basically how we can apply our knowledge of Western blotting to come up with conclusions for this Western blot so were told that we are looking at protein kinase C alpha and protein kinase C alpha has a molecular weight of 80 kilo daltons and that can be seen in the Western blot here so the question is telling us that well its asking us to make a conclusion and we can see that Western blot analysis of extracts of HeLa cause C 6 and and IH 3t3 cells using an anti pkc rabbit antibody or used to make this to yield this plot here so what the heck does this mean well this is just our these are our four cell lines these are just different cell lines it really doesnt matter actually because this question doesnt ask us anything about anything in particular about these you know specifics like it doesnt need any prior background knowledge all were doing is looking at this and coming up with a conclusion so what we can see straight
