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hi and welcome to my video series of pyrotechnics explained in five minutes where I explain the concept in biology in less than 5 minutes and if you havent yet subscribed to my channel please hit that subscribe button during installment well be talking about Western blot the Western blot is a technique which would allow us to detect our protein of interest in a pool of protein now nowadays it is like widely used as a diagnostic tool in many infectious disease which well be talking about and this is like HIV might be diagnosed using Western blot so Western blot has immense importance and in fundamental research Western blot is also very important so we would discuss all these aspects in this video so lets start how the Western blot works so in Western blot you have to take your cell lysate or protein extract or bacterial extract anything from where you want to get the protein source so you have to separate the protein along the molecular weight in a SDS page gel so the first degree
