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in this video were going to talk about the theory and practice of western blotting so were going to go through a little bit of why we do all the different steps that we do and then also talk about practically how to do them in the lab so the first thing i want to say about western blots is that they can be a little bit tricky just because they are so long they usually take two days to do with sort of the standard protocols and there are many many different steps and therefore many different points where something could go wrong and unfortunately with the western blot you dont really visualize your results until the very end and so if something does go wrong its hard to know and that is why a lot of people have trouble when they first start doing these but if you go step by step and you sort of troubleshoot every step as you go it is a pretty straightforward protocol and once you have some practice i think its very doable so just be very systematic think every step through and tak
