Editing WPT is fast and simple using DocHub. Skip downloading software to your PC and make changes with our drag and drop document editor in a few easy steps. DocHub is more than just a PDF editor. Users praise it for its convenience and powerful features that you can use on desktop and mobile devices. You can annotate documents, generate fillable forms, use eSignatures, and deliver documents for completion to other people. All of this, put together with a competitive price, makes DocHub the ideal option to blot sample in WPT files with ease.
Make your next tasks even easier by turning your documents into reusable templates. Don't worry about the protection of your records, as we securely keep them in the DocHub cloud.
Welcome to the Novus Visual Protocol Series. In this video we will learn how to perform all phases of a Western Blot using the most common methods for this assay. Before we can start preparing the blot we must first prepare our sample lysate. In this example we will prepare a protein lysate from cultured cells. Here we wash the cells twice with ice cold PBS and enough lysis buffer to cover the cells. The choice of lysis buffer depends largely upon the localization of your protein of interest. We scrape the cells and transfer the cell solution on a centrifuge tube placed on ice. In order to solubilize membrane bound proteins, we will require stronger extraction detergents compared to isolated cytoplasmic proteins. In this example we are using a standard RIPA buffer, which is a common buffer for obtaining maximum protein yield. While extracting proteins from all cellular localizations, it is very important to include protease inhibitors in your lysis buffer which will prevent degradation
