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Western blot is a technique to detect and analyze the protein of Interest it is based on the principle of antigen antibody interaction in the first step the gel is ran where the protein is basically separated on base of its molecular weight so among these protein present in the gel we need to find whether our protein of interest is present or not and that is detected using an antibody but first the content of the gel that means all the proteins are basically transferred into a pvdf membrane with Electro blotting technique then the membrane is incubated with primary antibody blocking solution and then the secondary antibody letamp;#39;s say the protein of interest is present so the primary antibody would detect that protein of Interest secondary board antibody would detect the primary antibody and a color reaction would be developed there are different modes of development like enzymatic chemiluminescent and fluorescent this is how a typical gel look like and there are wide variety of
