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hello in todayamp;#39;s tutorial video iamp;#39;ll be teaching you how to run a western blot so before i go ahead and show you the steps to performing a western law letamp;#39;s briefly talk about what it is so what is western blot technique basically itamp;#39;s a technique used to detect proteins and that can be from your choice of sample in my case youamp;#39;ll see that my samples are cells but you can isolate the proteins from tissue plants bacteria etc so how does it work briefly what western blot does is that it separates proteins by their molecular weight and so in order to do that we make use of the sodium diesel sulfate polyacrylamide gel electrophoresis um sds page for short so when youamp;#39;re in the lab very rarely will someone say the entire name so we refer to as an sds gel or a page gel and so what that does is that um the sodium diesel sulfide has a negative charge to it which masks the negative charge of our proteins so when weamp;#39;re running an sd
