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the overall goal of this procedure is to demonstrate the ease of performing a western blot when using appropriate products following sample preparation proteins are separated based on size and then transferred to a solid phase the proteins are then probed with antibodies and detected the use of proper protocols and high quality cost-effective reagents produce enhanced western blotting results hi iamp;#39;m jesse lujan r d scientist at emd chemicals in san diego california emd is an affiliate of merck kgaa in darmstadt germany today weamp;#39;re going to show you a western blotting procedure so letamp;#39;s get started the first step in western blotting is sample preparation begin sample preparation by pelleting the cells using low speed centrifugation such as five minutes at 2 500 times gravity following centrifugation drain the cell pellets well resuspend the cells in cytobuster protein extraction reagents using 150 microliters per 10 to the six cells incubate the sample at
