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the first step to a Great Western blot is preparing your sample correctly because the final result will never be a better quality than the starting material there are three main steps to preparing your sample lysis measurement of protein concentration and reduction and denaturation lysis essentially soluble eiseamp;#39;s the proteins from the cells or tissue using buffers and mechanical agitation all of this is done on ice and with the addition of fresh Prettyamp;#39;s inhibitors to the buffer because keep in mind that there are active protease enzymes in cells and tissues that can break down your sample if allowed the protocol is slightly different for cells or tissue samples but itamp;#39;s really the same concept homogenized the sample and lysis buffer and agitate then centrifuge and collect the supernatant once the samples are fully licensed entrer ssin you can use the Bradford assay or BCA si whichever method you prefer this will be important to know for the electrophoresis ste
