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Western blotting is an important technique used in cell and molecular biology for protein separation and detection it enables the separation and identification of a specific protein of interest from a complex mixture of proteins for example a cell lysate A typical Western blot technique includes the following steps separation by size, transfer of protein to a solid support and detecting target protein using antibodies to separate the macromolecules in a sample SDS-PAGE technique can be used The first step in the SDS-Page procedure is sample preparation So that, a loading buffer containing SDS, betamercaptoethanol bromophenol blue and glycerol is added to the protein samples the loading buffer is used in order to give all proteins present a uniform negative charge since proteins can be positively, negatively, or neutrally charged Once the loading buffer is added the samples are then heated to 95 degrees Celsius Proteins are large biomolecules, consisting of one or more long chains of am
