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this step-by-step western blotting video will show you how to get better results by doing exactly what you do today by using the ecl prime blotting reagent mike will take you step by step through western blotting giving you all the tips and tricks you need to get the best from amersham ecl prime hi amersham ecl prime is a great western blotting reagent it enables you to take minute quantities of proteins using very little of your precious antibody the intense signal it gives lasts an extremely long time adding flexibility to your western blotting workflow it works with almost all standard protocols for chemiluminescent reagents we find it better than all the other product options we have tried after completing the electrophoresis of your sample simply continue to use your own standard western blotting protocol there are three areas for you to consider before you start it is important that the sample is of good quality and that the gel is loaded optimally select the electrophore