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this is a gelbach store separating proteins through electrophoresis the gel itself is one millimeter thick between two glass plates the top of the gel has this castle-like appearance and we will be loading samples into the wells that are numbered here between one to ten to low the gel I use special tips for the micropipettes here you can see that itamp;#39;s got a very slender end and this will help me deliver my sample exactly where I want it to go in the gel this is the lemon a sample buffer so this has within it SDS which will help denature the protein and it has some glycerol to make it denser than water so it will sink into the wells Iamp;#39;m going to try to load 15 microliters in to lane number three this is my 15 microliters of loading dye the narrow pipette tip will fit between the two plates of glass so Iamp;#39;m inserting into slot number three and Iamp;#39;m slowly ejecting if I push too fast I may disturb the purple dye that looks very good you can see the purple dye
