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hello everyone welcome back to my channel excellent protein x in this tutorial video iamp;#39;m going to talk about the protocol or sample preparation for the western plot letamp;#39;s begin so first you have to take out your dish cell culture dish and you have to discard the media and then you have to put your duties on ice and remember that that enter all of the step you have to conduct on ice and the reagent you have to use all of the cold reagent and then wash with cold pvs and then add coal to deeper or np property lysis buffer in each oil usually for hundred microliter hipaa with proteus or phosphate is inhibitor for each oil of the six well split and that is for around one million cells and add protease or phosphorus inhibitor just before using the ripper to avoid the degradation of this phosphorus of this inhibitor and also you can you can adjust the volume of this river ing to your cell number if your cell number is less then you can use less volume of the reaper or if you n
