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Run at (S1) 80V, 20 min (S2) 100V, 1.5H (Speed of stacking gel amp;lt; resolving gel) Yes itamp;#39;s a squeaky door. This time itamp;#39;s a cat wailing. Ensure that entire sandwich is submerged in transfer buffer before closing sandwich Black side of cassette is facing the red side of the module Run at 120V, 1H