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western blotting what is it how does it work and why is it useful western blotting is used to detect a specific protein in a sample by combining gel electrophoresis and antibody recognition in a nutshell the samples proteins are first separated with the help of gel electrophoresis then they are transferred out of the gel and onto a surface of a membrane this membrane is then exposed to an antibody which has been selected for its quality to specifically bind to the target protein in addition a radioactive or chemical tag has also been added to the antibody for detection in the past on this channel we have discussed both immunoassays and sds page and as you can see western blotting really combines these two techniques into one even greater technique let us take a closer look at how western blotting is carried out first the sample is separated using sds page which separates the proteins ing to their molecular weight if you want to understand better how this technique works i will link the