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this is a guide of how you can perform the eliza test for the ACL kit it will give you visually all the information you need to do the tests including the use of the tara software all thorough test kit boxes are labeled with the content of the kit the expiration date and the kids lot number allow all the reagents and microplates to warm to room temperature which is 18 to 25 degrees Celsius by removing them from the kit box as demonstrated below this will take at least 30 minutes the kit contains a datasheet presenting the range of appropriate measurements that the calibrators and controls must fall within the units and raises change for each new lot prepare all necessary equipment to perform the test they are listed below prepare the 1x ACL wash buffer using a graduated cylinder at 900 milliliters of deionized water and 100 milliliters of 10x concentrated ACL wash buffer following the preparation of the 1x ACL wash buffer take the label from the concentrated 10x ACL wash buffer and pla