Blot questionaire in WRI

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Aug 6th, 2022
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How to blot questionaire in WRI

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foreign Dr Diane Lyme disease expert here and weamp;#39;re going to talk about the Western blot test now before we jump down that test and talk about some of the problems with the test I want to talk about a precursor task to that so most classically in conventional medicine people do start with the task called the Elisa test the standard of care is to run the Elisa people only move past the Elisa to the secondary test called the Western blot if the Elisa is positive now the problem with this is the one study showed that there was a 66 percent chance of a false negative from the Elisa test so many people have done this first round of testing they donamp;#39;t even make it to the second round of testing because they have a negative test and they actually do have Lyme disease so thatamp;#39;s really important to understand secondly the Western blot is a test where whatamp;#39;s essentially whatamp;#39;s happening is your blood is drawn and theyamp;#39;re looking for bands bands are

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A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. This mixture can include all of the proteins associated with a particular tissue or cell type.
To know how to analyze western blot data, Look for the sizes of the bands. These will be represented by a number, either followed by kDa or preceded by p. This is the size of the protein which has been detected and is the scale on which the proteins are separated in a Western blot.
The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract.
Western blotting (also called Protein Immunoblotting) is an analytical technique used to detect specific proteins in the given sample. It uses SDS-polyacrylamide gel electrophoresis (SDS-PAGE) to separate various proteins contained in the sample.
The Western blot test separates the blood proteins and detects the specific proteins (called HIV antibodies) that indicate an HIV infection. The Western blot is used to confirm a positive ELISA, and the combined tests are 99.9% accurate.
Blotting is the process of transferring macromolecules (proteins, nucleic acids, etc.) from a gel to the solid surface of an immobilised membrane to detect the molecules that have been transferred. Identification of specific sequences of DNA, RNA, and proteins is important for various studies in Molecular biology.
The Western blot test is used to confirm or contest a diagnosis of HIV or Lyme disease after an ELISA antibody test comes back positive or negative. Since the ELISA test sometimes produces false positives, a second test is needed to further the diagnosis.
However, the Western blot is no longer used, and today the ELISA test is followed by an HIV differentiation assay to confirm HIV infection.

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