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enzyme-linked immunoassay or Elisa is used to rapidly detect and quantify specific antigens or antibodies in a patientamp;#39;s serum first depending on the method being used either an antigen or a capture antibody is attached to the surface of a micro tighter plate then the patientamp;#39;s Serum is added if the antibody or antigen being tested for is in the serum it will bind detection of the target molecule is accomplished by adding antibodies that are linked to a colorimetric enzyme when the substrate is added a color change occurs this allows for the presence of the tested antigen or antibody to be assessed visually or Quantified using image analysis Western blotting is a laboratory technique that is used to confirm the presence of specific proteins in a sample first protein lysates are prepared and loaded onto different Lanes of polyacrylamide gel application of a charge pulls the proteins in the sample down toward the bottom of the gel larger proteins travel slowly through the
