Blot questionaire in NEIS

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How to blot questionaire in NEIS

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welcome back today weamp;#39;ll be talking about the blotting tests and I try to cover everything that youamp;#39;ll need to know for you step 1 exam before we jump into the mechanism youamp;#39;ll have to know that we use the blotting test to detect the presence of certain segments of DNA or detect the presence of certain gene expression or the presence of a specific protein and this is how this is done so weamp;#39;ll start off on the sample of DNA that may or may not contain the certain segments of DNA that you are looking for weamp;#39;ll use cleaving enzymes to take off one strand of the DNA and weamp;#39;ll add a radio-labelled primer that is specific to that DNA that we want for example if weamp;#39;re looking for HIV DNA weamp;#39;ll add label primers to that HIV DNA if that sample of DNA did in fact contain some HIV DNA this primer very specific for that HIV DNA will link to this segment and then we will be able to detect it now the process of detecting a specific prot

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What diseases can western blot detect?

Western blotting is frequently used for the confirmatory medical diagnosis of infectious diseases such as Lyme disease, HIV infection, bovine spongiform encephalopathy (BSE), hepatitis C infection, syphilis, inflammatory muscle conditions such as myositis, and certain autoimmune disorders (e.g., paraneoplastic disease)

What are the medical uses of Western blot?

Western blot is often used in research to separate and identify proteins. In this technique a mixture of proteins is separated based on molecular weight, and thus by type, through gel electrophoresis. These results are then transferred to a membrane producing a band for each protein.

What is blot test for?

The Western blot test separates the blood proteins and detects the specific proteins (called HIV antibodies) that indicate an HIV infection.

What does a Western blot detect?

Definition. Western blotting is a laboratory technique used to detect a specific protein in a blood or tissue sample. The method involves using gel electrophoresis to separate the samples proteins. The separated proteins are transferred out of the gel to the surface of a membrane.

For which disease Western blot is the confirmatory test?

A Western blot test is typically used to confirm a positive HIV diagnosis. During the test, a small sample of blood is taken and it is used to detect HIV antibodies, not the HIV virus itself.

What are the diagnostic applications of Western blot?

In recent medical field, Western Blot has a wide range of applications in medical diagnosis, such as the application of medical diagnosis for HIV (Human Immunodeficiency Virus) infection, BSE (Bovine Spongiform Encephalopathy, also known as mad cow disease), FIV (Feline Immunodeficiency Virus), HBV (Hepatitis B Virus

How do you remember blot tests?

The mnemonic to remember blotting techniques is SNoW DRoP S - Southern - DNA - D N - Northern - RNA - R O - Oooooo - Ooooo - O W - Western - Protein - P Blotting techniques illustration and mnemonic :) Theres another simpler one for all those who can speak the language Hindi..

What is a blot technique?

Blotting is the process of transferring macromolecules (proteins, nucleic acids, etc.) from a gel to the solid surface of an immobilised membrane to detect the molecules that have been transferred. Identification of specific sequences of DNA, RNA, and proteins is important for various studies in Molecular biology.

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