Blot question in NEIS in a few clicks

Aug 6th, 2022
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How to blot question in NEIS

5 out of 5
39 votes

hi weamp;#39;ve received a question on our western blotting forum regarding quantitative western blotting applications what is the difference between qualitative and quantitative western blotting hi anne marie could you explain this to us yeah of course uh qualitative western blotting when you perform that analysis youamp;#39;re only interested in seeing the presence or the absence of a specific protein of interest then you may roughly just want to check the levels of the protein in your sample but if youamp;#39;re more interested in in quantitative quantitative western blotting then you would like to put a figure on for example how much the protein has changed over time or how much protein you have in your sample could could you explain this a little further yeah i have two examples so first uh i have this uh qualitative wasnamp;#39;t bloating application and i have two clones here in this result and one is overexpressed and i just want to see that itamp;#39;s here and verify tha

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