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hi my name is David Borden Iamp;#39;m an RD staff scientist in protein analysis and weamp;#39;re going to go through the steps today of the traditional Western blot transfer okay so to get started letamp;#39;s talk about the things we need here first of all so weamp;#39;ve got our deionized water weamp;#39;ve got our transfer buffer thatamp;#39;s already made up into the appropriate formulation weamp;#39;ve got our new page gel weamp;#39;ve already run our protein samples on weamp;#39;ve got our pee PDF filter sandwich kit weamp;#39;ve got our sponges weamp;#39;ve got our blot module our power supply and a timer okay so letamp;#39;s get started so first of all we need to go ahead and start with our stack assembly so we take two of our sponges and dip them into some of the transfer buffer that Iamp;#39;ve already poured into this plastic container next we take what are our filter paper and submerge them both of them in the transfer buffer as well now what Iamp;#39;m going
