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in the instructions to authors for the Journal of biological chemistry they state authors should clearly explain whether signal intensity has a linear relationship with antigen loading what does this mean well in quantitative Western blot analysis the linear range of detection is the range of sample loading that produces a linear relationship between the amount of target on the membrane and the band intensity recorded by the detector within the linear range of detection band intensity should be proportional to the amount of target a change in target abundance should produce an equivalent change in signal response at the upper and lower ends of the linear range this proportional relationship is lost when attending global longer reflects the abundance of target and quantification is not possible quantitative Western blood analysis is only accurate if the target protein and the internal loading control can both be detected within the same linear range a range that must be determined exper
