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hi and thanks for making the switch to digital film with the C digit blot scanner before you get started scanning I want to give you a few quick tips to help make your first chemiluminescent 2 stands successful conveniently the maximum scan size of the C digit is about the size of a Mini Joe eight and a half by 10 centimeters and before you turn on your C digit be sure to image studio software on your computer a link to the image studio tutorial can be found in the same webpage where you click to view this video and here are the minimum computer requirements for image studio let me offer some quick tips for successful scanning on the C digit so that you get high quality high sensitivity images right from the start if youre already doing film then many of these tips will already be familiar to you because enzyme activity varies with temperature its important to always run your assay at the same temperature this is why for the best most consistent results your substrate should