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sds-page can be used to determine the molecular weight of protein relative to standards shown here is a typical SDS page gel after has been run and stained samples were loaded here at the top and they migrated down through the gel toward the positive electrode on both edges of the gel standards of known molecular weight were loaded shown here for example this very first band is 245 kilo daltons and it migrated 2 centimeters down into the gel this dart band is 135 killer Daltonamp;#39;s and it migrated 2.8 centimeters down into the gel in order to calculate the molecular weight of an unknown we can plot the log the log 10 of the molecular weight versus the distance that the standards migrated Iamp;#39;m going to show you how to do this in Excel and weamp;#39;re going to figure out how to calculate the molecular weight of this band here eh that migrated six point nine centimeters letamp;#39;s jump over to excel Iamp;#39;ve already tabulated the molecular weights of all events and th