Blot pecularity in zip

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Aug 6th, 2022

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How to blot pecularity in zip

4.9 out of 5

38 votes

okay so letamp;#39;s go through the steps of an eye plot so we have our eye blot apparatus here and letamp;#39;s go ahead and unwrap our anode stack put that on the device and weamp;#39;ve got our gel thatamp;#39;s ready to go here our new page gel that Iamp;#39;ve run some purified GFP and Novakamp;#39;s sharp pre-stained ladder okay then weamp;#39;re going to pre-wet our one filter paper put that on top then use our roller to roll out any bubbles now weamp;#39;re going to go ahead and put our cathode stack on top and then finish off with our sponge on top now we have seven minutes to wait okay that sound is the completion of our seven minute I blot transfer letamp;#39;s go ahead and take down the eye blot stack so first of all open the apparatus take off the sponge take off the top electrode take off the filter paper take off our new page gel and here is our PVDF membrane and behold our proteins of interest are Lisa we can say for now the the die fronts transferred as well a

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