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welcome everybody to my webinar today itamp;#39;s titled rethinking the traditional Western blot my name is John lisand and Iamp;#39;m a field application scientist with like Loree biosciences so for my talk today I want to talk about the incel Western assay I want to talk about what it is its benefits and how you can use it in the lab in your everyday experiments I also want to talk about a variation of the Insel Western assay which we call an onel Western assay after I go over the assays I then want to talk about a case study in which a group uses the inso Western assay to study Alzheimeramp;#39;s so what is the inso Western assay the inso Western assay begins with plating your cells in a microtiter plate these plates can range from from anything from a six W plate up to a 1536 W plate the One requirement for these plates is that theyamp;#39;re clear bottoms for laser detection at a later step now once youamp;#39;ve plated your cells you wait until theyamp;#39;re of confluency