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introducing EEB lot l1 the most efficient fast wet transfer system by gen script unpack your instrument and lay it on a flat surface and plug it into an outlet turn on the instrument to get it ready for operation first plug in one of the long tubes into the blue Inlet and connect the other end to the buffer bottle with the blue screw cap next do the same with the green Inlet and connect the other end to the water bottle with the green screw cap lastly connect the tube to the white Inlet and the other end to the waste bottle with the white screw cap letamp;#39;s remove the transfer cassette from channel a open the cassette and laid flat on the bench top remove the nitrocellulose membrane and place it into a tray containing the 1x nitrocellulose equilibration buffer remember if you are using PVDF make sure to wet the membrane in isopropanol first and then place it into the PVDF equilibration buffer open the gel using the cassette opening tool then place a gel in water open the d