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hello everyone so today Iamp;#39;m going to be showing you how we can take a stack of singular jell block images and convert them into a multiplex image for things like total protein normalization or to see the ratio of different protein bands hello everyone so today Iamp;#39;m going to be showing you how we can use the builtin create Multiplex function in ftic 1D to turn a stack of singular images into one multi Channel image now use case for this would be if you had a membrane that you had stripped and then reprobed with another protein uh or if youamp;#39;d taken a membrane that youamp;#39;ve already probed for one protein stripped it and then used um a total protein stain to perform normalization further down the line so to create Multiplex images within ftic 1D we open up ftic 1D and before inserting an image we get these options weamp;#39;ve got the option to open an image and the option to create a multiplex image so we want to go ahead and click create mul Multiplex and we