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revolutionizing protein blotting the seven-minute i-blot western blotting system the i-blot western blotting system that consists of the i-blot device and the i-blot transfer stacks does not require any liquid buffers and can easily be set up in less than two minutes the system uses the unique iblot gel transfer stacks that contain anode and cathode transfer buffers each i-blot bottom stack includes an integrated nitrocellulose or pvdf membrane for protein capture no liquid buffers means no mess and no fuzz the iblot western blotting system efficiently transfers proteins from polyacrylamide gels to nitrocellulose or pvdf membranes in seven minutes this remarkable time savings are achieved without compromising transfer quality using the standard 7-minute i-blot protocol you can obtain excellent transfer results with most of your samples however the time and voltages are adjustable so you can optimize the transfer of very small or very large proteins when needed i western blotting syste