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The i-Blot Western blotting system revolutionizes protein blotting with its innovative design consisting of the i-Blot device and transfer stacks. Setting up the system takes less than two minutes, and it eliminates the need for liquid buffers, resulting in a mess-free process. The unique i-Blot gel transfer stacks contain anode and cathode transfer buffers, and each bottom stack includes an integrated membrane for protein capture. In just seven minutes, proteins are efficiently transferred from gels to membranes without compromising quality. The standard protocol yields excellent results, but time and voltages can be adjusted for optimal transfer of proteins of varying sizes.