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This video tutorial demonstrates how to set up a polyacrylamide gel for wet tank transfer in a mini transplot cell. After removing the gel from the cassette, equilibrate it in transfer buffer. Pre-soak blotting paper and fiber pads, ensuring complete coverage. For nitrocellulose membrane, pre-wet it with transfer buffer; for pbdf membrane, pre-soak in 100% methanol. Create the blotting sandwich by placing the gel, pre-wet fiber pad, and blotting paper in a tray with transfer buffer. Carefully place the membrane on the gel to avoid air bubbles, ensuring even protein transfer for maximum efficiency.
