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Immunoblotting, also known as Western blotting, is a technique used to identify specific antigens within a protein sample using antibodies. This process involves transferring proteins separated by electrophoresis to nitrocellulose membranes and incubating them with primary and secondary antibodies. The antigens are then visualized using colored or fluorescent substrate reactions. The video tutorial provides a step-by-step demonstration of the immunoblotting procedure, including protein separation, blotting, immunoprobing, and visualization with chromogenic and chemoluminescent substrates.
