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This video covers the core content necessary for understanding blotting for the MCAT. The MCAT places a high importance on interpreting blots. The process of preparing a blot involves three main steps: running a sample on a gel to separate it by size, transferring the samples from the gel via blotting, and visualizing the sample using specific antibodies. An example provided is analyzing the levels of adenosine diphosphate and adenosine triphosphate in different cell line cytosols by running them on a gel, transferring to a blotting paper, and using markers to detect the molecules.
