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This video covers the core content of blotting for the MCAT, focusing on the interpretation of blots. The process involves running a sample on a gel, transferring the samples via blotting, and visualizing the sample using antibodies to detect specific molecules. For example, to measure ATP and ADP in different cell line cytosols, extract the cytosol, run it on a gel, transfer it to a blotting paper, and expose it to a marker designed to detect ATP and ADP. Finally, interpret the results from the blot.
