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[Music] Blotz it is a molecular biology technique that checks for the presence of a specific sequence of nucleotides or protein in a sample southern blot it is a method used to detect specific DNA sequence in a given DNA sample DNA samples are separated via electrophoresis on a gel the separated DNA is transferred to a filter the DNA is exposed to a label DNA probe that anneals to its complement the labeled probe is visualized locating the DNA sample of interest northern blot it is used to study gene expression by detection of RNA in a sample it is the same as southern blot except the sample is RNA and not DNA the probe remains DNA Western blot it is used to detect specific proteins it is similar to other types of blot but protein specific antibodies are used for detection after the proteins are electro phoretic Li separated and transferred to a filter a primary antibody is applied the excess primary antibody is washed off and secondary antibody is applied secondary antibody recognize
