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hello everyone in this video we will be discussing visualizing and analyzing western blot results we will be going over chromogenic chemoluminescent and fluorescent detection as well as important things to consider when analyzing your results first off we will discuss the chromogenic or colorimetric detection method cholemetric assays can be conducted through a horseradish peroxidase or an alkaline phosphatase system in both systems the membrane is incubated with the chromogenic substrate and the hrp or ap enzyme while incubating the enzyme will catalyze the substrates to produce a colored precipitate which become visible to the eye as the precipitate accumulates the substrates will appear as a color band where the antigen antibody complex is located color metric assays are beneficial due to their simplicity and lower costs as they do not require materials such as developers or x-ray films however these assays have low sensitivity and require picograms of protein next we will discuss
