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This video tutorial covers the core content necessary for understanding blotting for the MCAT. The focus is on interpreting blots, with a brief overview of the blotting method. Blotting involves three major steps: running a sample on a gel to separate it by size, transferring the samples to a special paper via blotting, and visualizing the sample using antibodies designed to detect specific molecules. For example, to determine the levels of adenosine diphosphate and adenosine triphosphate in different cell line cytosol, one would extract the cytosol, run it on a gel, transfer it to a blotting paper, and use antibodies to detect the molecules.
