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The PerkinElmer Lightning blot system offers fast electro blotting in 12 minutes or less, with no buffer preparation required. Proteins up to 250 kDa can be efficiently transferred without blow-through of low molecular weight proteins. To perform a Western blot, follow these steps: place the filter on the electro blotter, smooth the PVDF membrane on top, add the gel, connect to a power supply, and run at 24 volts. The transfer will be complete in 12 minutes or less. Disconnect and retrieve the PVDF blot for quantitative transfer.