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the perkinelmer lightning plot system provides fast electro blotting in 12 minutes or less and eliminates buffer preparation steps you can obtain efficient transfers of proteins up to 250 kilo Dalton's with no blow through of low molecular weight proteins to perform a Western blot using the Lightning blot system first open envelope 1 and place the filter on the Lightning blot electro blotter remove the overlay next open envelope to and smooth the PVDF membrane on top remove the overlay and place your gel on top of the stack you then open envelope 3 and place on top of the gel smooth and remove the overlay now you can connect the lightning blotter to a power supply and run a constant 24 volts the current will rise to about six hundred milliamps and gradually drop to about 300 milliamps by the end of the run transfer will be complete in about 12 minutes or less disconnect and retrieve the PVDF blot using the PerkinElmer lightening blot system we have demonstrated quantitative transfer o...