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in this video we will show you how to process and prepare your gel for western transfer after electrophoresis run your gel until the dye front docHubes the bottom of the gel then turn off the power supply and remove the power leads from the electrophoresis tank pour the running buffer out of the core electrode assembly so you can remove the gel cassette from the tank to remove the gel from the core assembly open each wing clamp from the core now remove the gel cassette next open up the cassette by using the gel opening lever insert the lever at the location of the four arrows printed on the cassette and lift or lower to crack open that corner once the four corners of the gel cassette have been cracked peel the two plates apart one method to remove the gel from the cassette is to invert the gel in a buffer filled tray alternatively slightly wet your gloves with buffer to minimize sticking and ripping then gently pick up the gel from the bottom and place it in a buffer filled tray