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hello welcome to watch creative proteomics video today we are going to briefly introduce Western blot Western blot was introduced in 1979 which is a commonly used method for protein analysis it can be used for qualitative and semi quantitative protein analysis for the accomplishment of the Western blot there are three elements separation of proteins by size transferring proteins to a solid support and marking proteins by primary and secondary antibodies for visualization sds-page allows protein samples to be separated and transferred to a solid support such as nitrocellulose Herpa live enlightened difluoride membrane the solid support can absorb proteins and keep its biological activity unchanged the membrane is treated with the primary antibody of the target proteins only the proteins to be studied and specifically bind to the primary antibody to form an antigen antibody complex after the primary antibody is washed and removed only the position of the target protein binds to the prima
