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in this video we explain how to use the signal accumulation mode exposure setting when imaging a chemiluminescent blot on a chemidoc imaging system the signal accumulation mode in image lab touch software provides the most film-like experience and will make intuitive sense if you are transitioning from film to digital imaging this mode produces a series of cumulative images of your blot and mimics taking several film images with increasing exposure time to use signal accumulation mode select the manual exposure setting estimate the shortest and longest exposure times that you want the system to acquire then enter the number of images that you want acquired during this interval for example letamp;#39;s enter a minimum exposure time of 5 seconds and a maximum of 30 seconds weamp;#39;ll take 5 images total the instrument will acquire images at approximately 5 11 18 24 and 30 seconds scan through the images to select your desired exposure time after determining the best exposure
