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hi everyone today we will discuss about Western blot membrane types and their uses so a little bit introduction to Western blotting western blotting or immunoblotting is a well-established and widely used technique for the detection and analysis of proteins the method is based on building and antibody protein complex we are specific binding of antibodies to proteins immobilized on a membrane and detecting the bound antibody with one of the several detection methods western blotting method was first described in 1979 and has since become one of the most commonly used methods in licensed research these are the types of membranes used in western blotting there are three types of membranes widely used in western blotting they are nitrocellulose membranes nylon membranes whole even held in fluoride membranes or the PVD of membranes let us look into the details of each of these first let us see a nitrocellulose membrane nitrocellulose membranes are available in pore size of point 4 5 micron