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foreign substances to be identified that is the target molecule in Northern blotting is RNA for southern blotting DNA and for Western proteins denaturation of the target molecule is done using formaldehyde in Northern blotting restriction enzymes in Southern blotting and sodium dodecile sulfate SDS in Western blotting the hybridization probe used in Northern blotting is target-specific RNA probe for southern blot Target specific ssdna probe is used in antibody probe used in Western blotting application of Northern blotting include gene expression profiling Southern blotting is used for DNA fingerprinting and genome mapping while Western blotting is useful for protein characterization identification expression analysis
